Determination of Triiodothyronine by RIA-MATT3 Kit
نویسندگان
چکیده
منابع مشابه
Determination of triiodothyronine in red blood cells by radioimmunoassay.
This study was undertaken to determine T3 content in red cells by radioimmunoassay. T3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell T3 content could be determined directly by radioimmunoassay of the lysate. After hemolysing red cells with an equal volume of distilled water, 0.4 ml of the hemolyzate was used for the assay. The red-cell T3 content was expres...
متن کاملSimplified radioimmunoassay for triiodothyronine: evaluation of a new commercial kit.
A new triiodothyronine (T3) radioimmunoassay kit offers simplicity of method, short incubation time, specificity, and reproducibility. 125I-labeled T3 and unknown serum (or standard) are added to alkaline buffered (pH 12.4) Sephadex G-25 columns; the T3 is freed of proteins with an alkaline buffer wash, antiserum is added, and the columns are incubated at room temperature for 2 h. "Free" hormon...
متن کاملTRIIODOTHYRONINE (T3) ENZYME IMMUNOASSAY TEST KIT Catalog Number: 6107205
Measurement of serum triiodothyronine concentration is generally regarded as a valuable tool in the diagnosis of thyroid dysfunction. This importance has provided the impetus for the significant improvement in assay methodology that has occurred in the last two decades. The advent of monospecific antiserum and the discovery of blocking agents to the T3 binding serum proteins has enabled the dev...
متن کاملSerum triiodothyronine determination in clinical use.
Two radioimmunassays for the determination of serum triiodothyronine (T3) were developed. The assay of T3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of T3 in serum from 117 patients requiring assessment of their thyroid status. In 53 subjects considered retrospectively not to have thyroid dysfunction nor to have bee...
متن کاملSensitive RIA for the specific determination of insulin lispro.
Insulin lispro is an insulin analog in which the primary sequence has been altered by the inversion of amino acids B28 and B29. To date, it has not been possible to specifically measure insulin lispro in the presence of endogenous insulin because of the high degree of homology between these peptides. However, the specific determination of insulin lispro offers advantages over quantifying total ...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1977
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.26.6_403